Background : The ABO Blood Group system was the first human Blood Group System to be discovered by Landsteiner in 1900. The second type of Blood Group System is the Rhesus system. There are only two Rh phenotypes such as Rh positive and Rh negative depending on whether Rh antigen is present on the red cell or not .The frequency of ABO and Rh phenotypes in different population has been extensively studied. Based on this the present study has been undertaken to assess the prevalence of Blood Group according to ABO blood group , Rh system and to correlate with their sex, category and religion and also to determine the predominated Blood Groups among them. Materials & Methods: The present study has been undertaken in the IInd semester students in the Hematology Lab of Department of Physiology. A total of 150 students were admitted through MP PMT 2012 , All India 2012, GOI seat and NRI seat of different categories but only 119 students participated in the study .15 were absent continuously due to their family problems 16 were absent in all the three turns when the practical was repeated . Hence they were excluded from the study group. Results: The Blood Group O had the highest prevalence in the II Semester students 37(31.09%) followed by blood group B 36 (30.25%). Conclusion: The commonest ABO blood group was O with Rh negativity of only 9(7.57%).