Background: Reaction time is defined as interval of time between presentation of stimulus and appearance of appropriate voluntary response in a person. Reaction time has physiological significance and is a simple and non - invasive test for peripheral as well as central neural structures. The measurement of simple reaction time has been used to evaluate the processing speed of central nervous system and the co-ordination between the sensory and motor systems in normal participants. Simple reaction time is an indirect index of processing capability of Central Nervous System. Materials and Methods: 52 healthy males and 52 healthy females [Mean age 27 5 years], who individually matched for gender and age, were included in the study, by using a response analyser to evaluate the sensori-motor performance. Results: Simple reaction time is the simplest model of measuring the function of the central nervous system. There were significant statistical differences [p<0.05] between males and females performance on sensori-motor measures. Their performances were expressed in mean ± standard deviation of the reaction time by using the student unpaired ‘t’ test. Conclusion: From these study it was concluded that the males might using a more complex strategy than females and were faster than females at aiming at a target, where as the females were more accurate or there may be modulation of neurotransmitter coupled with altered rate of impulse transmission due to fluctuation in the level of sex steroids hormones affecting the sensory, motor association and the processing speed at the central nervous system due to retention of sodium and water in females.